Geno2pheno [resistance] TESTVERSION ALT 3.4

Welcome to geno2pheno[resistance]. Detection of HIV-1 subtype A6 for all subtype A pol genes is provided by the beta version of the geno2pheno A6 subtyper at https://subtyping.geno2pheno.org The last sample sequence is of subtype A6 Etravirine and rilpivirine resistance predictions, as well as the scored mutations for these predictions, stem from rules-based drug-resistance interpretation models by HIV-GRADE. Rules-based drug-resistance models will be replaced with statistical models as soon as a sufficient number of genotype-phenotype pairs become available. Predictions for drugs other than rilpivine and etravirine are still based on statistical models. Default predictions have been altered to produce worst-case predictions. Specifically, only mutations resulting in the largest resistance factors will be used for prediction when ambiguities are present in the input sequence. Statistical models for predicting drug exposure have been implemented. Predictions performed with these models can be accessed by selecting the Drug Exposure tab.

Upon submitting an HIV-1 pol-gene DNA sequence below, the sequence will be aligned to the reference strain HXB2 and interpreted with several statistical models.

1. Identifier (optional)	Do not use patient names!
2. Pol-gene (PR and RT) nucleotide sequence:	upload from file (sequences in FASTA format, or single plain or FASTA sequence): or paste in:
3. Therapy-naïve tool:	enable therapy-naïve tool
4. Sequence ambiguities:	In case of ambiguities, only use mutations resulting in the largest resistance factor
5. Result layout options:	Alignment width: 30 60 90 120
6. Alignment:	Global Local Auto
7. Action:	Align and Predict Reset (Clear Session) Load sample sequences Switch to vertical layout

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You will make prediction № 341124. Service started December 13, 2000.