Robert J. Lipshutz, Ph.D.
Vice President, Corporate Development
Affymetrix, Inc.
3380 Central Expressway
Santa Clara, CA 95051
Abstract:
The Human Genome Project is
providing life science researchers with access to unprecedented
amounts of raw sequence data. To effectively harness this data
and apply it to biomedical research, therapeutic development,
clinical practice, and patient management, powerful new tools
for measuring gene expression, polymorphism discovery, and genotyping
are needed. GeneChip® probe arrays are powerful tools to meet
these requirements. Light-directed chemical synthesis is used
to generate miniaturized, high-density arrays of oligonucleotide
probes called GeneChip probe arrays. Application specific oligonucleotide
arrays have been used to rapidly scan known genes and discover
genetic variants, to detect the presence of known alternative
alleles, and to simultaneously measure the expression of thousands
of individual genes. An integrated GeneChip® system including
instrumentation and software has been developed for array hybridization,
fluorescent detection, and data acquisition and analysis. Experiments
demonstrating the effectiveness of these methods of genetic analysis
will be described as well as new bioinformatics challenges generated
by the new information.
References may be found at: www.affymetrix.com/technology/papers.html